Genetic modification of hematopoietic stem cells: methods and protocols / ed. by Ch.Baum. - New York: Humana Press, 2009. - xxi, 494 p.: ill. - (Methods in molecular biology; 506) (Springer protocols). - Incl. bibl. ref. - ISBN 978-1-58829-980-2; ISSN 1064-3745  Место хранения:   021 | Институт цитологии и генетики CO РАН | Новосибирск | Библиотека

Оглавление / Contents

Preface ........................................................ v Contributors ................................................ xvii 1 Immunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy Applications ..................... 1 Guillermo Guenechea, Jose C. Segovia, and Juan A. Bueren 2 Isolation of Human and Mouse Hematopoietic Stem Cells ....... 13 Yuk Yin Ng, Miranda R.M. Baert, Edwin F.E. de Haas, Karin Pike-Overzet, and Frank J.T. Staal 3 Murine Hematopoietic Stem Cell Transduction Using Retroviral Vectors .......................................... 23 Ute Modlich, Axel Schambach, Zhixiong Li, and Bernhard Schiedlmeier 4 Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-mediated Gene Transfer for Clinical Trials ........................... 33 Tulin Budak-Alpdogan and Isabelle Rivière 5 Short-Term Culture of Human CD34+Cells for Lentiviral Gene Transfer ............................................... 59 Francesca Santoni de Sio and Luigi Naldini 6 T Cell Culture for Gammaretroviral Transfer ................. 71 Sebastian Newrzela, Gunda Brandenburg, and Dorothee von Laer 7 Retroviral Transduction of Murine Primary T Lymphocytes ..... 83 James Lee, Michel Sadelain, and Renier Brentjens 8 Lentiviral Vector Gene Transfer into Human T Cells .......... 97 Els Verhoeyen, Caroline Costa, and Francois-Loic Cosset 9 DNA Transposons for Modification of Human Primary T Lymphocytes .............................................. 115 Xin Huang, Andrew Wilber, R. Scott McIvor, and Xianzheng Zhou 10 Retroviral Gene Transfer into Primary Human Natural Killer Cells ............................................... 127 Evren Alici, Tolga Sutlu, and M. Sirac Dilber 11 Lentiviral Vector-Mediated Genetic Programming of Mouse and Human Dendritic Cells .................................. 139 Renata Stripecke 12 In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells ................................................. 159 Dao Pan 13 In Vivo and Ex Vivo Gene Transfer in Thymocytes and Thymocyte Precursors ....................................... 171 Oumeya Adjali, Amelie Montel-Hagen, Louise Swainson, Sophie Marty, Rita Vicente, Cedric Mongellaz, Chantal Jacquet, Valérie Zimmermann, and Naomi Taylor 14 Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells ................. 191 Axel Schambach, William P. Swaney, and Johannes C.M. van der Loo 15 Knock-Down of Gene Expression in Hematopoietic Cells ....... 207 Michaela Scherr, Letizia Venturing and Matthias Eder 16 The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations ............................. 221 Rainer Löw 17 Detection of Replication Competent Retrovirus and Lentivirus ................................................. 243 Lakshmi Sastry and Kenneth Cornetta 18 Release Testing of Retroviral Vectors and Gene-Modified Cells ...................................................... 265 Diana Nordling, Anne Kaiser, and Lilith Reeves 19 Copy Number Determination of Genetically-Modified Hematopoietic Stem Cells ................................... 281 Todd Schuesler, Lilith Reeves, Christofvon Kalle, and Elke Grassman 20 Tissue Procurement for Molecular Studies Using Laser- Assisted Microdissection ................................... 299 Ulrich Lehmann and Hans Kreipe 21 Leukemia Diagnosis in Murine Bone Marrow Transplantation Models ..................................................... 311 Zhixiong Li, Ute Modlich, and Anjali Mishra 22 Humanized Mouse Models to Study the Human Haematopoietic Stem Cell Compartment ....................... 331 Dominique Bonnet 23 Canine Models of Gene-Modified Hematopoiesis ............... 341 Brian С. Beard and Hans-Peter Kiem 24 Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Individual Integration Clones in Different Samples .................... 363 Manfred Schmidt, Kerstin Schwarzwaelder, Cynthia С. Bartholomae, Hanno Glimm, and Christofvon Kalle 25 Retroviral Insertion Site Analysis in Dominant Haematopoietic Clones ...................................... 373 Olga S. Kustikova, Ute Modlich, and Boris Fehse 26 Tracking Gene-Modified T Cells In Vivo ..................... 391 Alessandra Recchia and Fulvio Mavilio 27 DNA Microarray Studies of Hematopoietic Subpopulations ..... 403 Karin Pike-Overzet, Dick de Ridder, Tom Schonewille, and Prank J.T. Staal 28 Quantification of Genomic Mutations in Murine Hematopoietic Cells ........................................ 423 Hartmut Geiger, David Schleimer, Kalpana J. Nattamai, and Jan Vijg 29 Proteomics Studies After Hematopoietic Stem Cell Transplantation ............................................ 437 Eva M. Weissinger, Petra Zürbig, and Arnold Ganser 30 Spectral Karyotyping and Fluorescence In Situ Hybridization of Murine Cells ...................... 453 Cornelia Rudolph and Brigitte Schlegelberger 31 Database Setup for Preclinical Studies of Gene-Modified Hematopoiesis .............................................. 467 Brenden Balcik, Elke Grassman, and Lilith Reeves 32 The US and EU Regulatory Perspectives on the Clinical Use of Hematopoietic Stem/Progenitor Cells Genetically Modified Ex Vivo by Retroviral Vectors ..................... 477 Carolyn A. Wilson and Klaus Cichutek Index ......................................................... 489