Reich E. High-performance thin-layer chromatography for the analysis of medicinal plants (New York; Stuttgart, 2007). - ОГЛАВЛЕНИЕ / CONTENTS
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ОбложкаReich E. High-performance thin-layer chromatography for the analysis of medicinal plants / E.Reich, A.Schibli. - New York; Stuttgart: Thieme, 2007. - xv, 264 p.: ill. - Incl. bibl. ref. - Ind.: p.259-264. - ISBN 1-58890-409-1; ISBN 978-1-58890-409-6 (US); ISBN 3-13-141601-7; ISBN 978-3-13-141601-8 (GTV)
 

Оглавление / Contents
 
Foreword ....................................................... xi
Preface ...................................................... xiii
Acknowledgments ................................................ xv

1  Introduction ................................................. 1
   Analysis of Herbal Drugs ..................................... 1
   What Is Chromatography? ...................................... 6
   What Is TLC? ................................................ 11
   Modern TLC .................................................. 19
2  Theoretical Concepts ........................................ 22
   General Chromatographic Principles .......................... 22
   Special Aspects of TLC ...................................... 33
   The Stationary Phase ........................................ 42
   The Mobile Phase ............................................ 48
   The Chromatographic Result in TLC ........................... 55
   Densitometry ................................................ 61
3  Practical Aspects of Modern TLC ............................. 67
   Sample Preparation .......................................... 68
   Selecting the Stationary Phase .............................. 73
   Sample Application .......................................... 92
   Chromatogram Development .................................... 97
   Derivatization ............................................. 110
   Visualization and Documentation of the Chromatogram ........ 114
   Densitometry ............................................... 116
   Reporting and Record Keeping ............................... 121
   TLC Software ............................................... 125
   Standardization ............................................ 125
   Practical Recommendations .................................. 127
4  Typical Applications in Herbal Analysis .................... 129
   Why HPTLC? ................................................. 129
   HPTLC Analysis of Raw Material ............................. 132
   HPTLC Analysis of Herbal Drug Preparations and Products .... 160
5  Method Development ......................................... 175
   Theoretical Aspects ........................................ 175
   Method Development in Practice ............................. 179
6  Validation ................................................. 193
   General Information ........................................ 193
   Validation of Qualitative HPTLC Methods .................... 206
   Validation of Quantitative HTPLC Methods ................... 210
   The Final Word ............................................. 214

List of Abbreviations ......................................... 215

Glossary of TLC Terms ......................................... 218

Appendixes
   A Definitions .............................................. 224
   В Standard Operating Procedure for HPTLC of Herbal Raw
     Materials and Preparations ............................... 227
   С Resources ................................................ 231
   D Common Mobile Phases for the Screening of Unknown
     Herbal Drugs ............................................. 233
   E Most Common Nonspecific Derivation Reagents for
     Analysis of Herbal Drugs ................................. 234
   F Worksheet ................................................ 238
   G Validation of Method for Identification of Eleuthero by
     HPTLC Fingerprint ........................................ 240

Index ......................................................... 259


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